John M. Shelton

John M. Shelton, B.Sc.
Senior Research Scientist
Science & Business Manager
Texas A & M University '86
TBAALAS Technical Presenter Award, 1989
Donald W. Seldin Award, 2005

Research Expertise

As Technical Supervisor and Manager of the Molecular Pathology Core
Lab, I specialize in staining for mRNAs, retroviridae, and proteins by
in-situ hybridization, immunolocalization, and histochemistry; laser-
capture microdissection; digital image processing and analysis; and
photomicrography for client and collaborative research endeavors.

I draw on twenty-plus years of technical and lab management experience with
light and electron microscopy, histology, molecular biology, cell culture,
clinical pathology, and lab animal/plant science.

I work primarily in the lab's microscopy and imaging facility located
in the Moss Heart and Reynolds Cardiovascular Centers, augmenting

research efforts investigating muscle development and heart disease.
Additionally, I assist the lab's long standing research oriented
histology and molecular pathology collaborations with Howard Hughes
Medical Institute, the Division of Comparative Medicine, and the
Departments of Internal Medicine, Pathology, Physiology, Molecular
Biology, Biochemistry, Cell Biology, and Microbiology.

PUBLICATIONS

-IRF-1 promotes inflammation early after ischemic acute kidney injury. Wang, Y, John, R, Chen, J,
Richardson, JA, Shelton, JM, Bennett, M, Zhou, XJ, Nagami, GT, Zhang, Y, Wu, QC, Lu, CY.
J Am Soc Nephrol In-Press, 2009.

-Hypercalcemia of malignancy with simulatneous elevation in serum parathyroid hormone-
related peptide and 1,25-dihydroxyvitamnin D in a patient with metastatic renal cell carcinoma.
Shivnani, SB, Shelton, JM, Richardson, JA, Maalouf, NM. Endocr Pract 15(3): 234-239, 2009.

-Thymosin beta4 mediated PKC activation is essential to initiate the embryonic coronary
developmental program and epicardial progenitor cell activation in adult mice in vivo.
Bock-Marquette, I, Shrivastava, S, Pipes, GC, Thatcher, JE, Blystone, A, Shelton, JM,
Galindo, CL, Melegh, B, Srivastava, D, Olson, EN, DiMaio, JM. J Mol Cell Cardiol
46(5):728-728, 2009.

-Hand2 controls osteoblast differentiation in the branchial arch by inhibiting DBA binding
of Runx2. Funato, N, Chapman, SL, McKee, MD, Funato, H, Morris, JA, Shelton, JM,
Richardson, JA, Yanagisawa, H. Development 136(4): 615-625, 2009.

-Autophagy is an adaptive response in desmin-related cardiomyopathy. Tannous, P, Zhu, H,
Johnstone, JL, Shelton, JM, Rajasekaran, NS, Benjamin, IJ, Nguyen, L, Gerard, RD,
Levine, B, Rothermel, BA, Hill, JA. Proc Nat Acad Sci USA 105(28): 9745-9750, 2008.

-Intracellular protein aggregation is a proximal trigger of cardiomyocyte autophagy.
Tannous, P, Zhu, H, Nemchenko, A, Berry, JM, Johnstone, JL, Shelton, JM, Miller, FJ,
Rothermel, BA, Hill, JH. Circulation 117(24): 3070-3078, 2008.

-Myosin regulatory light chain phosphorylation attenuates cardiac hypertrophy.
Huang, J, Shelton, JM, Richardson, JA, Kamm, KE, Stull, JT. J Biol Chem 283(28):
19748-19756, 2008.

-Protein kinase D1 stimulates MEF2 activity in skeletal muscle and enhances muscle
performance. Kim, MS, Fielitz, J, McAnally, J, Shelton, JM, Lemon, DD, McKinsey, TA
Richardson, JA, Bassel-Duby, R, Olson, EN. Mol Cell Biol 28(11): 3600-3609, 2008.

-Requirement of protein kinase D1 for pathological cardiac remodeling. Fielitz, J,
Kim, MS, Shelton, JM, Qi, X, Hill, JA, Richardson, JA, Bassel-Duby, R, Olson, EN.
Proc Nat Acad Sci USA 105(8): 3059-3063, 2008.

-Deletion of hexose-6-phosphate dehydrogenase activates the unfolded protein response
pathway and induces skeltal myopathy. Lavery, GG, Walker, EA, Turan, N, Rogoff, D,
Ryder, JW, Shelton, JM, Richardson, JA, Falciani, F, White, PC, Stewart, PM,
Parker, KL, McMillan, DR. J Biol Chem 283(13): 8453-8461, 2008.

-Overexpression of pyruvate dehydrogenas kinase 4 in heart perturbs metabolism and
exacerbates calcineurin-induce cardiomyopathy. Zhao, G, Jeoung, NH, Burgess, SC,
Rosaaen-Stowe, KA, Inagaki, T, Latif, S, Shelton, JM, McAnally, J, Bassel-Duby, R,
Harris, RA, Richardson, JA, Kliewer, SA. Am J Physiol Heart Circ Physiol 294(2):
H963-H943, 2008.

-The Down syndrome critical region protein RCAN1 regulates long-term potentiation and
memory via inhibition of phosphatase signaling. Hoeffer, CA, Dey, A, Sachan, N, Wong, H,
Patterson, RJ, Shelton, JM, Richardson, JA, Klann, E, Rothermel, BA. J Neurosci 27(48):
13161-13172, 2007.

-Myosin accumulation and striated muscle myopathy result from the loss of muscle RING
finger 1 and 3. Fielitz, J, Kim, MS, Shelton, JM, Latif, S, Spencer, JA, Glass, DJ,
Richardson, JA, Bassel-Duby, R, Olson, EN. J Clin Invest 117(9): 2486-2495, 2007.

- Histone deacetylase degradation and MEF2 activation promote the formation of
slow-twitch myofibers. Potthoff, MJ, Wu, H, Arnold, MA, Shelton, JM, Backs, J,
McAnally, J, Richardson, JA, Bassel-Duby, R, Olson, EN. J Clin Invest 117(9):
2459-2467, 2007.

-Cardiac autophagy is a maladaptive response to hemodynamic stress. Zhu, H, Tannous, P,
Johnstone, JL, Kong, Y, Shelton, JM, Richardson, JA, Le, V, Levine, B, Rothermel, BA,
Hill, JA. J Clin Invest 117(7): 1782-1793, 2007.

-Loss of muscle-specific RING-finger 3 predisposes the heart to cardiac rupture
after myocardial infarction. Fielitz, J, van Rooij, E, Spencer, JA, Shelton, JM,
Latif, S, van der Nagel, R, Bezprozvannay, S, de Windt, L, Richardson JA,
Bassel-Duby, R, Olson, EN. Proc Nat Acad Sci USA 104(11): 4377-4382, 2007.

-MEF2C transcription factor controls chondrocyte hypertrophy and bone development.
Arnold, MA, Kim, Y, Czubryt, MP, Phan D, McAnally, J, Qi, X, Shelton JM,
Richardson, JA, Bassel-Duby, R, Olson, EN. Dev Cell 12(3): 377-389, 2007.

-Reactive oxygen species impair sympathetic vasoregulation in skeletal muscle
in angiotensin II-dependent hypertension. Zhao, W, Swanson, SA, Ye, J, Li, X,
Shelton, JM, Zhang, W, Thomas, GD. Hypertension 48(4): 637-643, 2006.

-Cytoglobin is a stress-responsive hemoprotein that is expressed in developing and
adult brain. Mammen, PP, Shelton, JM, Ye, Q, Kanatous, SB, McGrath, AJ,
Richardson, JA, Garry, DJ. J Histochem Cytochem 54(12): 1349-1361, 2006.

-Regulation of antibacterial defense in the small intestine by the nuclear bile
acid receptor. Inagaki, T, Moschetta, A, Lee, YK, Peng, L, Zhao, G, Downes, M, Yu,
RT, Shelton, JM, Richardson, JA, Repa, JJ, Mangelsdorf, DJ, Kliewer, SA.
Proc Nat Acad Sci USA 103(10): 3920-3925, 2006.

-Stem cells and their derivatives can bypass the requirement of myocardin for
smooth muscle gene expression. Pipes, GC, Sinha, S, Qi, X, Zhu, CH, Gallardo, TD,
Shelton, JM, Creemers, EE, Sutherland, L, Richardson, JA, Garry, DJ, Wright, WE,
Owens, GK, Olson, EN. Dev Biol 288(2): 502-513, 2005.
COVER MANUSCRIPT

-Maladaptive role of IL-6 in ischemic acute renal failure. Kielar, ML, John, R,
Bennett, M, Richardson, JA, Shelton, JM, Chen, L, Jeyarajah, DR, Zhou, H,
Chiquett, B, Nagami, GT, Lu, CY. J Am Soc Nephrol 16(11): 3315-3325, 2005.

-Centronuclear myopathy in mice lacking a novel muscle-specific protein kinase
transcriptionally regulated by MEF2. Nakagawa, O, Arnold, M, Nakagawa, M,
Hamada, H, Shelton, JM, Kusano, J, Harris, TM, Childs, G, Campbell, KP,
Richardson, JA, Nishino, I, Olson, EN. Gen and Dev 19(17): 2066-2077, 2005.
COVER MANUSCRIPT

-Temporal and spatial expression of liver receptor homologue-1 (LRH-1) during
embryogenesis suggests a potential role in gonadal development. Hinshelwood, MM,
Shelton, JM, Richardson, JA, Mendelson, CR. Dev Dyn 234: 159-168, 2005.

-Transcriptional regulation of aromatase in placenta and ovary. Mendelson, CR,
Jiang, B, Shelton, JM, Richardson, JA, Hinshelwood, MM.
J Steroid Biochem Mol Biol 95(1-5): 25-33, 2005.

-Calcineurin is necessary for the maintenance but not embryonic development
of slow muscle fibers. Oh, M, Rybkin, II, Copeland, V, Czubryt MP, Shelton, JM,
van Rooij, E, Richardson, JA, Hill, JA, De Windt, LJ, Bassel-Duby, R, Olson, EN,
Rothermel, BA. Mol Cell Biol 25(15): 6629-6638, 2005.

-Functional characterization of mRDH11 as a retinol dehydrogenase involved
in dark adaptation in vivo. Kasus-Jacobi, A, Ou, J, Birch, DG, Locke, KG,
Shelton, JM, Richardson, JA, Murphy,AJ, Valenzuela, DM, Yancopoulos, GD,
Edwards, AO. J Biol Chem 280(21): 20413-20420, 2005.

-Genomic regions that mediate placental cell-specific and developmental
regulation of human CYP19 (aromatase) gene expression in transgenic mice.
Kamat, A, Smith, ME, Shelton , JM, Richardson, JA, Mendelson, CR.
Endocrinology 146(5): 2481-2488, 2005.

-HIF-2{alpha} regulates murine hemapoietic development in an erythropoietin-
dependent manner. Scortegagna M, Ding K, Zhang Q, Oktay Y, Bennett MJ,
Bennett M, Shelton JM, Richardson JA, Moe O, Garcia JA. Blood
105(8): 3133-3140, 2005.

-Transcriptional coactivator PGC-1alpha controls the energy state and contractile
function of cardiac muscle. Arany, Z, He, H, Lin, J, Hoyer, K, Handschin, C,
Toka, O, Ahmad, F, Matsui, T, Chin, S, Wu, P, Rybkin, II, Shelton, JM, Manieri, M,
Cinti, S, Schoen, FJ, Bassel-Duby, R, Rosenzweig, A, Ingwall, JS, Spiegelman, BM.
Cell Metab 1(4): 259-271, 2005. COVER MANUSCRIPT

-A role for the apoptosis inhibitory factor AIM/Sp-alpha/Api6 in
atherosclerosis development. Arai, S, Shelton, JM, Chen, M, Bradley, MN,
Castrillo, A, Bookout, AL, Mak, PA, Edwards, PA, Mangelsdorf, DJ, Tontonoz, P,
Miyazaki, T. Cell Metab 1(3): 201-213, 2005.

-Critical roles of guanylyl cyclase B receptor in ednochondral ossification
and development of female reproductive organs. Tamura N, Doolittle LK,
Hammer RE, Shelton JM, Richardson JA, Garbers DL. Proc Natl Acad Sci USA
101(49): 17300-17305, 2004.

-Mice lacking calsarcin-1 are sensitized to calcineurin signaling and show
accellerated cardiomyopathy in response to pathological biomechanical stress.
Frey N, Barrientos T, Shelton JM, Frank D, Rutten H, Gehring D, Kuhn C,
Lutz M, Rothermel B, Bassel-Duby R, Richardson JA, Katus HA, Hill JA,
Olson EN. Nat Med 10(12): 1336-1343, 2004.

-Histone deacetylase 4 controls chondrocyte hypertrophy during skeletogenesis.
Vega RB, Matsuda K, Oh J, Barbosa AC, Yang X, Meadows E, McAnally J,
Pomajzl C, Shelton JM, Richardson JA, Karsenty G, Olson EN. Cell 119(4):
555-566, 2004. COVER MANUSCRIPT

-Regulation of hyaluronan expression during cervical ripening. Straach KJ,
Shelton JM, Richardson JA, Hascall VC, Mahendroo MS. Glycobiology
15(1): 55-65, 2004.

-TRPC3 channels confer cellular memory of recent neuromuscular activity.
Rosenberg P, Hawkins A, Stiber J, Shelton JM, Hutcheson K, Bassel-Duby R
Shin DM, Yan Z, Williams RS. Proc Natl Acad Sci USA 101(25): 9387-9392, 2004.

-Identification of acylooxyacyl hydrolase, a lipopolysaccharide-detoxifying
enzyme, in the murine urinary tract. Feulner JA, Lu M, Shelton JM,
Zhang M, Richardson JA, Munford RS. Infect Immun 72(6): 3171-3178, 2004.

-Normal development and fertility of knockout mice lacking the tumor
suppressor gene LRP1b suggest functional ompensation by LRP1. Marschang P,
Brich J, Weeber EJ, Sweatt JD, Shelton JM, Richardson JA, HammerRE, Herz J.
Mol Cell Biol 24(9): 3782-3793, 2004.

-Loss of NFAT5 results in renal atrophy and lack of tonicity-responsive
gene expression. Lopez-Rodriguez C, Antos CL, Shelton JM, Richardson JA,
Lin F, Novobrantseva TI, Bronson RT, Igarashi P, Rao A, Olson EN.
Proc Natl Acad Sci USA 101(8): 2392-2397, 2004.

-Ischemia-reperfusion induces G-CSF gene expression by renal medullary
thick ascending limb cells in vivo and in vitro. Zhang Y, Woodward VK,
Shelton JM, Richardson JA, Zhou XJ, Link D, Kielar ML, Jeyarajah, Lu CY.
Am J Physiol Renal Physiol 286(6): F1193-F1201, 2004.

-Essential role of STAT3 in body weight and glucose homeostasis. Cui Y,
Huang L, Elefteriou F, Yang G, Shelton JM, Giles JE, Oz OK, Pourbahrami T
Lu CY, Richardson JA, Karsenty G, Li C. Mol Cell Biol 24(1): 258-269, 2004.

-Multiple organ pathology, metabolic abnormalities and impaired homeostasis
of reactive oxygen species in Epas1 -/- mice. Scortegagna M, Ding K, Oktay Y,
Gaur A, Thurmond, F, Yan LJ, Marck BT, Matsumoto AM, Shelton JM, Richardson JA,
Bennett MJ, Garcia JA. Nat Genet 35(4): 331-340, 2003.

-Disruption of PPT2 in Mice Causes an Unusual Lysosomal Storage Disorder
with Neurovisceral Features. Gupta P, Soyombo AA, Shelton JM,
Wilkofsky IG, Wisniewski KE, Richardson JA, Hofmann, SL.
Proc Nat Acad Sci USA 100: 12325-12330, 2003.

-Expression of LRH-1 and SF-1 in the mouse ovary: localization in different
cell types correlates with differing function. Hinselwood MM, Repa JJ,
Shelton JM, Richardson JA, Mangelsdorf DJ, Mendelson CR.
Mol Cell Endocrinol 207(1-2): 39-45, 2003.

-Characterization of mouse short-chain aldehyde dehydrogenase (SCALD),
an enzyme regulated by SREBPs. Kasus-Jacobi A, Ou J, Bashmakov YK,
Shelton JM, Richardson JA, Goldstein JL, Brown MS.
J Biol Chem 278(34): 32380-32389, 2003.

-Alterations in slow-twitch muscle phenotype in transgenic mice overexpressing
the Ca2+ buffering protein parvalbumin. Chin ER, Grange RW, Viau F, Simard AR,
Humphries C, Shelton J, Bassel-Duby R, Williams RS, Michel RN.
J Physiol 547(Pt 2): 649-663, 2003.

-Control of facial muscle development by MyoR and capsulin. Lu JR, Bassel-Duby R,
Hawkins A, Chang P, Valdez R, Wu H, Gan L, Shelton JM, Richardson JA,
Olson EN. Science 298(5602): 2378-2381, 2002.

-Neuroglobin, a Novel Member of the Globin Family, is Expressed in Focal
Regions of the Brain. Mammen, PPA, Shelton JM,, Goetsch, SC, Williams,
SC, Richardson JA, Garry, MG, and Garry, DJ. J Hist Cyt 50(12): 1591-1598, 2002.

-Activated Glycogen Synthase-3 Beta Suppresses Cardiac Hypertrophy In Vivo.
Antos CL, McKinsey TA, Frey N, Kutschke W, McAnally J, Shelton JM,
Richardson JA, Hill JA, and Olson EN. Proc Nat Acad Sci USA 99(2) 907-912,
2002.

-Activation of MEF2 by Muscle Activity is Mediated Through a Calcineurin-
Dependent Pathway. Wu H, Rothermel B, Kanatous S, Rosenberg P, Naya FJ,
Shelton JM, Hutcheson KA, DiMaio JM, Olson EN, Bassel-Duby R,
and Williams RS EMBO 20(22): 6414-6423, 2001.

-Disruption of PPT1 or PPT2 causes neuronal ceroid lipofuchsinosis
in knockout mice. Gupta, P, Soyombo, AA, Atashband, A, Wisinewski, KE,
Shelton, JM, Richardson, JA, Hammer, RH, and Hoffman, SL
Proc Nat Acad Sci USA 98(24) 13566-13571, 2001.

-Functional and Molecular Adaptations in Skeletal Muscle of Myoglobin-
Mutant Mice. Grange, RW, Meeson, A, Chin, E, Lau, KS, Stull, JT,
Shelton, JM, Williams, RS, and Garry, DJ Am J Physiol Cell
Physiol 281: C1487-C1494, 2001.

-Myocyte-Enriched Calcineurin-Interacting Protein, MCIP1, Inhibits
Cardiac Hypertrophy In-Vivo. Rothermel, BA, McKinsey, TA,
Vega, R, Nicol, RL, Mammen, PPA, Yang, J, Antos, CL,
Shelton, JM, Bassel-Duby, R, Olson, EN, and Williams, RS
Proc Natl Acad Sci USA 98: 3328-3333, 2001.

-Adaptive Mechanisms That Preserve Cardiac Function in Mice Without
Myoglobin. Meeson, A, Radford, N, Shelton, JM,
Mammen, PPA, DiMaio, JM, Hutcheson, KA, Kong, Y, Elterman, J,
Williams, RS, and Garry, DJ Cir Res 88(7): 713-720, 2001.

-Cardiac-specific LIM protein FHL2 modifies the hypertrophic response
to b-adrenergic stimulation. Kong, Y, Shelton, JM, Rothermel, B
Li, X, Richardson, JA, Bassel-Duby, R, Williams, RS
Circulation 103: 2731-2738, 2001. COVER MANUSCRIPT

-Cyclosporine A-induced hypertension involves synapsin in renal sensory
nerve endings. Zhang, W, Li, J-L, Hosaka, M, Janz, R, Shelton, JM,
Albright, GM, Richardson, JA, Sudhof, TC, Victor, RG
Proc Natl Acad Sci USA 97: 9765-9770, 2000.

-Interactions of the Low Density Lipoprotein (LDL) Receptor Gene
Family with Cytosolic Adaptor and Scaffold Proteins Suggest Diverse
Biological Functions in Cellular Communication and Signal Transduction.
Gotthardt, M, Trommsdorff, M, Shelton, JM, Richardson, JA
Herz, J. J Bio Chem 275: 25616-25624, 2000.

-MEF2 Responds to Multiple Calcium-Regulated Signals in the Control of
Skeletal Muscle Fiber Type. Wu, H, Naya, FJ, McKinsey, T,
Mercer, B, Shelton, JM, Chin, ER, Bassel-Duby, R
Olson, EN, Williams, RS. EMBO 19(9): 1963-1973, 2000.

-Cytochrome C Deficiency Causes Embryonic Lethality and
Attenuates Stress Induced Apoptosis. Li, K, Li, Y
Shelton, JM,Richardson, JA, Wang, X
Williams, RS Cell 101: 389-399, 2000.

-Microsomal Triglyceride Transfer Protein Expression during Mouse
Development. Shelton JM, Lee MH, Richardson JA, and Patel SB
J Lip Res 41(4): 532-537, 2000. COVER MANUSCRIPT

-Stimulation of Slow Skeletal Muscle Fiber Gene Expression by
Calcineurin. Naya FJ, Mercer B, Shelton JM, Richardson JA,
Williams, RS, Olson EN J Bio Chem 275(7): 4545-4548,
2000.

-Reeler/Disabled-like Disruption of Neuronal Migration in Knockout
Mice Lacking the VLDL Receptor and ApoE Receptor 2. Trommsdorff M.,
Gotthardt M., Hiesberger T., Shelton J.M., Stockinger W., Nimpf J.,
Hammer R.E., Richardson J.A., and Herz J. Cell 97: 689-701, 1999.
COVER MANUSCRIPT

-A Calcineurin-Dependent Transcriptional Pathway Controls Skeletal Muscle
Fibertype. Chin, E., Olson, E.N., Richardson, J.A., Yang, Q., Humphries, C.,
Shelton, J.M., Wu, H., Zhu, W., Bassel-Duby, R., and Williams, R.S.
Gen and Dev 12: 16; 2499-2509, 1998. COVER MANUSCRIPT

-Cardiac Failure in Transgenic Mice with Myocardial Expression of Tumor
Necrosis Factor-a (TNF). Debora Bryant, Lisa Becker, James Richardson,
John Shelton, Fatima Franco, Ronald Peshock, and Brett Giroir.
Circulation 97: 14; 1375-1381, 1998.

-Molecular Characterization of Two Mammalian bHLH-PAS
Domain Proteins Selectively Expressed in the Central
Nervous System. Yu-Dong Zhou, Mary Barnard, Hui Tian,
Xu Li, Huijun Z. Ring, Uta Francke, John Shelton, James
Richardson, David W. Russell, and Steven L. McKnight.
Proc Natl Acad Sci USA 94: 2; 713-718, 1997.

-Temporospatial Expression of the Small HSP/aB-Crystallin
in Cardiac and Skeletal Muscle During Mouse Development.
Ivor J. Benjamin, John Shelton, Daniel J. Garry, and
James A. Richardson. Dev Dyn 208: 75-84, 1997.
COVER MANUSCRIPT

-Continuous Contractile Activity Induces Fiber Type Specific Expression of
HSP70 in Skeletal Muscle. P. Darrell Neufer, George A. Ordway, Gregory A.
Hand, John M. Shelton, James A. Richardson, Ivor J. Benjamin, and R.
Sanders Williams. Am J Physiol 271 (Heart Circ Physiol 40): C1828-C1837,
1996.

-Developmental Regulation of Cytochrome Oxidase Subunit VIa Isoforms in
Cardiac and Skeletal Muscle. W.J. Parsons, R.S. Williams, J.M. Shelton,
Y. Luo, D.J. Kessler, and J.A. Richardson. Am J Physiol 270 (Heart Circ
Physiol 39): H567-H574, 1996.

Page Maintained by: John Shelton, Senior Research Scientist, john.shelton@utsouthwestern.edu
Page last modified: April 12, 2009

[UT Southwestern] [Cardiology] [JAR-Mole Path Core]