Richardson Molecular
Pathology Core

Jim Richardson
John Shelton
Iman Abdeshahian
Jesse Morris
Sierra Porter
Chase Guy
Jonathan Guevara
Melanie Weiler




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CORE MISSION
Facilitate morphologic and histologic assessment of murine transgenic and knockout phenotypes for fundamental and clinical cardiovascular research as well as other basic / clinical sciences utilizing expertise in gross pathology, routine histology, in-situ hybridization, immunohistochemistry, laser-capture microdissection, photomicrography, and image analysis

REYNOLDS AND MOSS HEART CARDIOVASCULAR CENTERS PROJECTS

Skeletal Muscle Fibertype Specificity Transcriptional Pathway Studies
- In-vitro and in-vivo investigation of skeletal muscle fibertype remodeling by transgenic and knockout modulation of the MCIP1-calcineurin-NFAT-MEF2 signaling pathway
- Investigation of fibertype remodeling by transgenic and knockout modulation other calcium dependent signalling pathways, including parvalbumin, calsarcin 1 and 2, myoglobin and other proteins

Calcineurin Interaction Studies
- Investigation of MCIP1, MCIP2, and other molecules directly associated with calcineurin by characterization of transgenic and knockout phenotypes

Thoracic Aortic Banding Studies
- Investigation of cardiac disease models associated with acute hypertension
- Investigation of alternate cardiac cell death pathways resulting from acute hypertension

Gene Therapy Technology Studies
- Evaluation of "Microbubble" gene delivery technology using histologic endpoints
- Proof of principle investigation of bio-polymer-matrix implantation
- Evaluation of vascular stent gene delivery technology

Stem Cell Viability and Regeneration Studies
- Proof of principle and functional investigation for repopulation of injured myocardium and skeletal muscle with reporter gene expressing stem cell lineages

Globin Developmental and Cyto-Protection Studies
- Characterization of embryonic and neonatal expression of the cardiac, skeletal muscle, and neuronal proteins; myoglobin, cytoglobin, and neuroglobin
- Characterization of the partially viable myoglobin knockout phenotype and its response to cardiotoxin ablation


HAMON CENTER and HOWARD HUGHES MEDICAL INSTITUTE AND
OTHER COLLABORATIVE PROJECTS

Novel Gene Discovery Studies
- Ongoing pilot characterizations of yet undescribed genes involved in cardiac, skeletal muscle, neural crest development and regulation, and other organ systems
- Past discoveries and characterizations include: myogenin, scleraxis, FHL-2, EVEC, CRP-1 and -2, myo-R, HRT-1, -2, and-3, capsulin (pod), NKX2.5, myocardin, SCALD, TRPC3, NPAS-1 and -2, MCIP-1 and -2, EPAS-1 and -2, TBX5, BOP, and others

Experimental Heart Failure Studies
- Characterization of the calcineurin cardiac hypertrophy model, its cyclosporin A rescue, and phenotypes of cross breeding with inhibitor overexpression mice
- Characterization of other single and combinatorial genes involved in cardiac hypertrophy and dilated cardiomyopathy
- Investigation of ultrastructural causes of cardiac hypertrophy and dilated cardiomyopathy

Orexin Ligand and Recptor Studies
- Localization of the hypothalmic appetite regulating ligand / receptor and their protein or steroid antagonists
- Characterization of the narcolepsy model phenotype resulting from orexin receptor-2 knockout

CYP19 (aromatase) Expression Studies
- General analysis of promoter sequences responsible for placental specific expression using HGH reporter gene in-situ hybridization
- Detailed analysis of promoter sequences driving placental spongiotrophoblastic specifc expression
- Characterization of aromatase and other related steroidogenic factors during gonadal maturation and gonadal physiologic states

Endothelin Cascade Developmental Studies
- Temporospatial study of expression of embryonic endothelium determinants involved in the production of endothelin
- Characterization of the various knockout phenotypes

Renal Homeostasis Gene Characterization Studies
- Temporospatial characterization of renal ischemia response genes
- Temporospatial characterization of renal acidosis response genes

Brain Development and Neuronal Migration Studies
- Characterization of lipoprotein expression patterns in the developing embryonic and neonatal brain
- Characterization of phenotypes for lipoprotein mutant mice and multiple mutant crosses










The Journal of Histochemistry and Cytochemistry


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Page Maintained by:John Shelton, Senior Research Scientist, john.shelton@utsouthwestern.edu
Page last modified: May 1, 2008

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