Molecular Pathology Core
Description of Services
and Fees

The Molecular Pathology Core functions as a collaborative service center for the performance of routine
and custom histology to UT Southwestern research investigators/clinicians, as well research investigators
at other institutions, on a fee for service basis. A description of services and associated fees is listed below.


Fee Structure
Effective May 18, 2007

Abbreviated Schedule of Services and Associated Fees
PROCEDURE NOTES UNIT FEE
Grossing placing tissues in cassettes per cassette $2.00
Machine Paraffin Processing and Embedding automated w/o lacZ, w/o alk phos per cassette $6.00
Paraffin Sectioning and Routine H&E Stain single 5µm section on specimen slide w/ H&E staining per slide $6.50
Paraffin Sectioning and Massons Trichrome Stain single 5µm section w/ trichrome stain for collagen per slide $23.25
Paraffin Sectioning for Investigator Performed IHC single 5µm section on silanated slide per slide $5.00
Paraffin Sectioning and ISH; Analytical single 5µm section w/ radioisotopic ISH; 1-probe per slide $49.75


WET TISSUE HANDLING MICROTOMY & HISTOLOGY MICROTOMY; SECTIONING ONLY HISTOLOGY; STAINING & OTHER PROCEDURES MOLECULAR PATHOBIOLOGY IMAGING SUPPLIES
Comprehensive Schedule of Services and Associated Fees
PROCEDURE NOTES UNIT FEE
Grossing placing tissues in cassettes per cassette $2.00
Decalcification formic acid or EDTA per cassette $8.00
Machine Paraffin Processing and Embedding automated w/o lacZ, w/o alk phos per cassette $6.00
LacZ Paraffin Processing and Embedding Completion automated w/ manual lacZ finish per cassette + $3.00
Manual Paraffin Processing and Embedding manual f/ alk phos, f/ fine samples per sample $20.00
Complex Special Paraffin Embedding >3 tissues per cassette or grossing at embedding per cassette + $5.00
Cryoembedding isopentane supercooled or bulk gfp per embed $6.50
Wet Tissue Dissection Technical Assistance perfusion, bleeding, other per hour $55.00
PROCEDURE NOTES UNIT FEE
Paraffin Sectioning and Routine H&E Stain single 5µm section on specimen slide w/ H&E staining per slide $6.50
Paraffin Sectioning of Rumen and Routine H&E Stain single 5µm section of intestinal content on specimen slide w/ H&E per slide $8.50
Paraffin Sectioning and Special Stain; Facile single 5µm section w/ facile special stain (see stain classes) per slide $16.00
Paraffin Sectioning and Special Stain; Intermediate single 5µm section w/ intermediate special stain (see stain classes) per slide $23.25
Paraffin Sectioning and Special Stain; Complex single 5µm section w/ complex special stain (see stain classes) per slide $39.50
Paraffin Sectioning and ISH; Analytical single 5µm section w/ radioisotopic ISH; 1-probe per slide $49.75
Paraffin Sectioning and ISH; Screening single 5µm section w/ radioisotopic ISH; up to 6-probes per slide $148.50
Paraffin Sectioning and Core Performed IHC single 5µm section w/ core repertoire IHC per slide $34.00
Cryosectioning and Enzyme Histochemistry single 8µm cryosection w/ ATPase staining per slide $47.50
Cryosectioning and Oil Red-O Staining single 8µm cryosection w/ ORO staining per slide $19.00
PROCEDURE NOTES UNIT FEE
Paraffin Sectioning for Investigational IHC single 5µm section on silanated slide per slide $5.00
Core Library Paraffin Embryo Section; Specific Anat staged E8.5 - E15.5 unstained section on Vectabond slide; RNA per slide $22.00
Core Library Paraffin Tissue Section; Organ Library use of blocks from core mouse tissue library; RNA per slide + $2.00
Cryosectioning unstained 8µm frozen section on silanated slide per slide $8.00
Additional Cryosectioning Level; Serial or Interval additional 8µm levels on same slide per level $5.50
RNAse-free Section Mounting for Investigational ISH single 5µm section on Vectabond slide; RNA per slide + $1.00
Additional Paraffin Sectioning Level; Serial additional 5µm serial levels on same slide per level + $0.50
Additional Paraffin Sectioning Level; Interval additional 5µm other interval levels on same slide per level + $1.50
Additional Paraffin Sectioning; Complex SSOSS additional serial levels mounted on separate slides per level + $1.50
Additional Paraffin Sectioning; Additional Blocks multiple blocks on same slide per block + $4.00
Vibratomy albumin embedding and through sectioning per block $105.00
Sled Microtomy stage freezing and through sectioning per 1/4 series $80.00
PROCEDURE NOTES UNIT FEE
Deparaffinization dewaxing of slides to water, PBS per slide $1.50
Counterstaining and Coverslipping H&E, NFR, or other staining and coverslipping per slide $2.00
Coverslipping aqueous or dehydration and permount coverslipping per slide $1.50
Pathologist Review and Report non-collaborative pathologist effort per hour $200.00
PROCEDURE NOTES UNIT FEE
PCR Gendertyping soon to be available TBD $ TBD
Laser Microdissection; RNA soon to be available TBD $ TBD
Laser Microdissection; DNA soon to be available TBD $ TBD
Laser Microdissection; Protein/Enzyme soon to be available TBD $ TBD
RNA Determination soon to be available TBD $ TBD
PROCEDURE NOTES UNIT FEE
Use of Common Imaging Systems scheduled use of systems in NB11.204 per hour no charge
Core Assisted Use of Advanced Imaging Systems scheduled use of systems in NB11.108 per hour $40.00
Morphometrics & Graphics quantitation and figure preparation per hour $40.00
Dye-Sub Prints raster and postscript printing on Kodak XLS engine per page $8.00
ITEM NOTES UNIT FEE
Slide Storage Flats delivery of completed slides in 20-count flats each $18.50
Slide Storage Boxes delivery of completed slides in 50-count boxes each $18.50
Slide Storage Boxes delivery of completed slides in 100-count boxes each $18.50
Slide Mailers delivery of completed slides in 4-count mailers each $1.00


Special Stain Classes
CATEGORY STAINS
Sectioning & Facile Special Stain
$16.00 per slide
Cresyl-Echt Violet (Nissl)
Luxol Fast Blue (Myelin)
Periodic-Acid Schiff
Toluidine Blue
Congo Red (Amyloid)
Alcian Blue (pH2.5)
Picrosirius Red (Collagen Typing)
Rubeanic Acid
Sectioning & Intermediate Special Stain
$23.25 per slide
Masson's Trichrome
Gram Stain
Hart's Elastic (Elastin)
PAS-Fuelgen
Acid-Fast Bacilli/FITE
Von Kassa (calcium)
Iron Stain
Giemsa
Safranin-O (cartilage)
Sectioning & Complex Special Stain
$39.50 per slide
Gordon & Sweet (reticulin)
Grockett Methanamine Silver
Steiner
Fontanna Masson
Luxol Fast Blue (Myelin)/Cresyl Echt Violet (Nissl) Combo
Mayer's Mucicarmine

Page Maintained by:John Shelton, Senior Research Scientist, john.shelton@utsouthwestern.edu
Page last modified: May 1, 2007

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